![]() Fans will have to wait until The Flash arrives in theaters on June 16, 2023.Īs for Billy Batson and his family, Shazam! Fury of the Gods hits theaters on March 17. ![]() However, another rumor states she was removed from the project. Newly revealed Funko Pop! figures show Princess Diana among all the other characters in the movie. While Gal Gadot's future as Wonder Woman looks bleak, she at least has a strong chance of also appearing in The Flash. Fast-forward to the 1980s as Wonder Womans next big screen adventure finds her riding lightning across the sky, donning wings of gold, and chasing a dream. The big cameo from the first Shazam! film, Superman, didn't get the same privilege, as Henry Cavill was nowhere to be seen. A mature woman who usually looks into her plump breasts is a bad woman who is witn. ![]() Some of the best wonder woman cosplay nude xxx videos in HD format can be found on . Wonder Woman 1984 saw Cheetah gain Dianas power, but while her journey ended in defeat, a comic story took things in a more ambitious direction. Despite her third film being canceled, it looks like the actress isn’t done with the DCU yet. Wonder woman cosplay nude Best Porno Videos. Sandberg teased that it might very well be Gadot. However, more recently, director David F. Wonder Woman 1984 PG-13 2020, Action/Adventure, 2h 31m 58 Tomatometer 448 Reviews 73 Audience Score 2,500+ Verified Ratings What to know critics consensus Wonder Woman 1984 struggles with. What's Wonder Woman Doing in Shazam?ĭespite not being able to see her face, the above footage showing off Wonder Woman sure does look like Gal Gadot in her iconic costume.įans were originally thrown off the scent when an official press release for the movie actually revealed that actress Taylor Cahill will be playing "Fake Wonder Woman". The last time the Amazonian was seen on screen, she was her new vibrant get-up in Wonder Woman 1984. Some previous footage showed off Wonder Woman's bracers, magical staff in hand. This scene has actually been shown off before. DC Studiosįor those who can't spot her, she is standing to the right of the group, possibly holding that important glowing staff emphasized throughout the various promotional material. The cropped screencaptures are low-resolution, and it is hard to make out any details, but it seems clear that Wonder Woman is standing among what looks to be the Shazam Family. for Shazam! Fury of the Gods gave fans a better look at Wonder Woman in the film. Director Patty Jenkins suggested that the major theme of this film is the. A New Look at Wonder WomanĪ new official TV spot released by Warner Bros. With Fury of the Gods right around the corner, the rush of promotional material is giving fans additional bits and pieces of Gal Gadot's rumored Wonder Woman appearance in the movie. Princess Diana's part in the movie was actually first witnessed earlier last year in footage shown to fans in attendance at CinemaCon 2022, though it was much later when the general audience got to see part of the same snippet. For those excited for Wonder Woman's cameo in Shazam! Fury of the Gods, new footage has been uncovered that teases the superhero's role in the Zachary Levi-led film. Starring: Alex Delescu, Amr Waked, Amreen N.
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![]() Perhaps these weren’t features meant to be focused on during this first experience with the game, but it would still be nice to have these systems explained to the point that they are easy to use when the game is released. Same with the crafting station back at the hub. There is a note that mentions the use of other elements, but it didn’t properly explain how to use them. I hope that there will be a better guidance system with the finished game, even if it is as simple as a marking on a map I can pull up. It seemed like there was more to do, but I couldn’t find where to go after clearing out the initial section the game guided me to. Despite my efforts and going everywhere I could see or think of, I couldn’t figure out where to go after clearing the amount of the game I did in my First Look video at the top of this write-up. So, it is weird that immediately following a solid tutorial setup, the game would have a fairly poor guidance system to help players move forward in the right direction. The tutorial for this game is pretty well designed and gives the proper amount of information for players to understand the controls. One of which I look forward to trying out and that is the multiplayer that this game will eventually offer. This is where you will find access to aspects such as customization, level-up system, and many other interactions or menu-type items. Here you are safe and all of your energy, health, and battery stash restock automatically. Other than the action in the field, you will find beacons that can be used to teleport back to the main hub. Good thing is, you can heal yourself with your energy bar even while fighting, so pulling off clutch moments. ![]() It is the best time to spam the melee attacks and do as much damage as possible because you will take the least amount yourself. If you come across a stronger enemy or get ambushed, you can activate your armor reactor which would put a shield around your body to help absorb some of the damage you are going to take. Sometimes it took more than half health damage with the ranged weapon before it was safe to move in though, so you’ll need to consider your approach, especially with new enemies. After I got the health down to about half and they were slowed by the ice effect, I would finish them off with melee. You can use either small or large shots to take off damage, but your melee attacks will do the most. When it came to fighting, I liked to start with my ice gun as it would slow enemies down and lower their health before getting close. ![]() It is easy to get overrun and ambushed, so make sure you take it slow through new areas as well. Enemies will have a mix of ranged and close-ranged attacks, so being careful is a must. In the demo, I faced off with a few different enemies, each having their own attacks. There is going to be more elements available at a later point in the game so you can switch it up to fight specific enemies. This demo gave access to the standard ax weapon and an ice-based elemental gun. Essentially, you can heal yourself by consuming some of your energy bar, refill your energy bar by consuming a battery, hold up a plasma shield to block, fight with a melee weapon, and fight with a shooting weapon. Starting off, this game will have you run into little red circles that will provide tutorial tips on how to play. ![]() Little bit surprised the game is supported on 3 different platforms what a clap I guess. There is also the game lets you roaming figure out places yet it’s not that open but kinda giving the games more compelling in which the gameplay. Enjoying his summer and navigating risky situations. I feel like both are only has slight very slight change in the story only not the characters, the characters pretty much same. Prince Of Suburbia In Prince of Suburbia PC Game You Can Play The main character is an 18 year old male. Prince of Suburbia is a kinetic (linear) adult visual novel with only one thing on its mind. In the first game you will see there are 2 modes that you can choose which is incest or not. I cannot be more cricitze into the story because of you know people taste are different but for me who is already play a lot of visual novel with already bunch of stories already read this one is quite bit very normal that make me no longer focus or get me into the stories. Discover the growing collection of high quality. ![]() His main goal in this industry is to bring enjoyable games to the fans. TheOmega is a web developer by trade that has been involved in the adult gaming world for the past few years. I often feel the game like this is only the arts that I can only tell which is good or not the second one is the story. Watch Prince Of Suburbia porn videos for free, here on . We are a two-person indie game studio currently developing two adult visual novels, Prince of Suburbia and A Shot in the Dark. Thus so far I cannot really tell how it suit with my preference, I played a lot already with game made by using renpy (which is most of them is indeed a visual novel-ish type of game). Download the best sex games, adult visual novels, XXX kinetic novels and lewd RPGs made by the top adult developers on FAP-Nation. I have band all Rose to the back of the tank until I know what I'm going to do with them.Īre you looking for data for a project or behavior study? I can see about getting them to split. I have been trying to get my main Rose to split so it can become smaller. Bulb and Long Tentacle Anemones require a minimum of Power Compact lighting, but T5 or Metal Halide Lighting is preferred by most. Some other common names it is known by are the Bulb-Tip Anemome, Purple Base Anemone, Maroon Anemone, and Yellow Tipped Long Tentacle Anemone. More difficult anemones are Sebae, Ritteri or Magnificient and Carpet Anemones these anemones should be avoided by beginners and do not make a good first anemone. Long time aquarists still commonly refer to it as the Ritteri Anemone. The tentacles reach 50mm in length, may be. Both the tentacles and oral disc of White Corn Anemone or Ritteri Sand Anemone are brown or purplish. I have been feeding them Silver Sides about 2 -3 times a week. It used to be known primarily as the Ritteri Anemone because for years it was classified as Radianthus ritteri. White Corn Anemone, also known as Sand Anemone, Carpet Anemone, Corn Anemone, Ritteri Anemone, Saddle Tip Anemone, the White Beaded Anemone, is a species of sea anemone in the family Stichodactylidae. Av Nossa senhora do Sabará 2179, 04685-005 San Paolo. Each anemone is about 75% of the original. Você pode conferir melhor os ideais e metodologia de trabalho da AgapeAquarismo Atendemos toda. At first I thought the base was in a hole and found 2 openings. I was looking at my tank one evening and notice a split between 2 buds. ![]() I didn't noticed what the Ritteri was doing prior to splitting. I have been having a war with the main bud as it likes to sneak over to my sps and sting it. I returned it to the LFS where it eventually died. This anemone did not like this and moved all over the tank. When I was a newbie, I had a Ritteri under 175 W MH with 2X110 W VHO in a 100 g tank. They need lots of light and lots of water movement. I also have 3 Rose in the tank that seems to be on a splitting spree. The Ritteri Anemone or Magnificent Sea Anemone is characterized by a flared oral disc which reaches between 20 and 50 centimeters in diameter but in some. magnifica is right under a 400 W MH, only about 4 inches under the water. I really don't test the water much unless the SPS don't look happy. Anemones are considered to be semi aggressive as they will eat fish and crustaceans, although most animals have a built in understanding of the. Well I can't say that my tank have the "PERFECT" parameters. The Ritteri anemone or magnificent anemone is by far one of the most stunning, the long tapered tentacles and movement provide something iconic to the reef. ![]() It turns web data scattered across pages into structured data that can be stored in your local computer in a spreadsheet or transmitted to a database. It can be difficult to build a web scraper for people who don’t know anything about coding. Luckily, there is data scraping software available for people with or without programming skills. Also, if you're a data scientist or a researcher, using a web scraper definitely raises your working effectiveness in data collection. Here is a list of the 30 most popular free web scraping software. I just put them together under the umbrella of software, while they range from open-source libraries, browser extensions to desktop software and more.īest 30 Free Web Scraping Tools 1. Who is this for: developers who are proficient at programming to build a web scraper/web crawler to crawl the websites. Why you should use it: Beautiful Soup is an open-source Python library designed for web-scraping HTML and XML files. It is the top Python parser that has been widely used. If you have programming skills, it works best when you combine this library with Python. Who is this for: Professionals without coding skills who need to scrape web data at scale. This web scraping software is widely used among online sellers, marketers, researchers and data analysts. Why you should use it: Octoparse is a free for life SaaS web data platform. ![]() With its intuitive interface, you can scrape web data within points and clicks. It also provides ready-to-use web scraping templates to extract data from Amazon, eBay, Twitter, BestBuy, etc. If you are looking for a one-stop data solution, Octoparse also provides web data service. Or you can simply follow the Octoparse user guide to scrape website data easily for free. Who is this for: Enterprises with budget looking for integration solutions on web data. Why you should use it: Import.io is a SaaS web data platform. It provides a web scraping solution that allows you to scrape data from websites and organize them into data sets. They can integrate the web data into analytic tools for sales and marketing to gain insight. Who is this for: Enterprises and businesses with scalable data needs. Why you should use it: Mozenda provides a data extraction tool that makes it easy to capture content from the web. They also provide data visualization services. ![]() It eliminates the need to hire a data analyst. And Mozenda team offers services to customize integration options. Who is this for: Data analysts, marketers, and researchers who lack programming skills. Why you should use it: ParseHub is a visual web scraping tool to get data from the web. You can extract the data by clicking any fields on the website. It also has an IP rotation function that helps change your IP address when you encounter aggressive websites with anti-scraping techniques. Why you should use it: CrawlMonster is a free web scraping tool. ![]() It enables you to scan websites and analyze your website content, source code, page status, etc. Who is this for: Enterprise looking for integration solution on web data. Why you should use it: Connotate has been working together with Import.io, which provides a solution for automating web data scraping. It provides web data service that helps you to scrape, collect and handle the data. Who is this for: Researchers, students, and professors. Why you should use it: Common Crawl is founded by the idea of open source in the digital age. It provides open datasets of crawled websites. It contains raw web page data, extracted metadata, and text extractions. Who is this for: People with basic data requirements. Why you should use it: Crawly provides automatic web scraping service that scrapes a website and turns unstructured data into structured formats like JSON and CSV. They can extract limited elements within seconds, which include Title Text, HTML, Comments, DateEntity Tags, Author, Image URLs, Videos, Publisher and country. ![]() We should note here that stomata in the basal parts mature once needles completely emerge from buds, which is typical for conifers, but the number of stomatal rows is set in the early stages of needle growth inside buds. The presence of stomata in phase B2 and the development of stomatal complexes in phase B3 indicate that the differentiation of stomata began after the initiation of longitudinal needle growth but before the needles emerged from the bud in the late B2 phase. In physiological and eco-physiological studies, this distributional pattern is often used and can be valuable also in the case of very young needles, especially when we consider that the morphological and anatomical development of needles and leaves is correlated with the physiological status of a plant. cembra were arranged in the same pattern as those in mature needles. The stomata present in phase B3 (when brachyblasts remained entirely enveloped by the parts constituting the sheath) in P. Stomata on growing pine needles in the B3 phase were described by Dvořák and Štokrová. In dormant winter buds, stomata are not visible on needle primordia, but they can be observed on young needles after they emerge from buds. The differentiation and development of stomatal complexes are closely tied to the need for gas exchange in growing needles. Most of the water absorbed from the soil is released through the stomata, and therefore, stomata closing and opening are strictly connected to a plant’s response to environmental factors. The stomata are anatomical gates that allow plants to take up CO 2, which is necessary for photosynthesis, while they retain a plant’s ability to control water loss by transpiration. In gymnosperms, stomatal complexes are arranged in rows, and the rows present an age gradient, which is a consequence of divisions of the intercalary meristem located in the basal part of a needle. Stomata emerge from the protodermal cells as a protoderm differentiates into the epidermis. Some of its cells, stomatal progenitor cells (meristemoid mother cells), produce a stomatal complex. Protoderm, which differentiates into the epidermis, covers the surface of needle primordium. Stomata differentiate from protodermal cells. In needles, the morphology of stomata is of special interest. Numerous, arranged in rows, visible Florin rings Numerous, arranged in rows, clearly visible Florin rings Numerous, big, pointed teeth covering needle tip Numerous, rounded in upper part, pointed on tip Morphological traits in chosen Pinus species in the B2 and B3 phases of bud development. These phenological stages were defined by him as follows: B0-dormant buds B1-buds are swollen B2-buds elongate and disjunction of bud scales makes the shoot surface visible B3-emergence of brachyblasts, which remain entirely enveloped by the parts constituting the sheath B4-emergence of needles and B5-disjunction of needles from the same fascicle. Debazac divided the process of growth into six phases, from B0 to B5, and proposed a classification consistent with bud morphology. During the expansion of long-shoot buds into mature shoots, some phenological stages can be distinguished. The length of a shoot is, to a large extent, determined by the number of short-shoot primordia initiated in the previous season, which are present in the bud. The growth of a shoot, defined as “predetermined” (buds contain all the primordia for the following season and, thus, the numbers of leaves and future lateral buds are already predetermined in the bud), can be completed in a relatively short time after dormancy breaking in spring. ![]() In a long-shoot bud, there are primordia of each structure of the future mature shoot. Short-shoot buds typically contain all the needle primordia formed in autumn before bud dormancy. Short shoots are covered by live cataphyll scales (typically 8–11), and the needle primordia of these shoots are tightly enclosed by them. To the best of our knowledge, this is the first study on the micromorphology of very young needles in representatives of the genus Pinus. These observations could be useful in pine systematics but also in palaeobotanical or physiological studies. strobus, Florin rings were also observed. In B3 for all the species, numerous stomata were visible. strobus, their size and density along the margin decreased basipetally. The teeth on the margin in all the species were pointed. strobus was the needle tip slightly rounded. In the B3 phase, needle tips were pointed. nigra) near the needle tips and were arranged in rows. Stomata became visible in the late B2 phase ( P. Teeth were also visible on the margins in P. In B2, needle tips were rounded or pointed, depending on the species. uncinata) were analyzed at phenological stages B2 and B3 (according to Debazac). Using a scanning electron microscope, the micromorphologies of needle primordia and the young needles of seven pine species ( Pinus cembra, P. ![]() Use these tips to spice up the conversation with friends and coworkers on Slack, iMessage, WhatsApp, Facebook, Twitter, etc. You have a witty, and creative brain, I hope this tutorial helps you convert those funny thoughts into a meme for many others to laugh and enjoy. This is how you can create custom memes on your iPhone or iPad. These are the best free sites to create memes online: Start by visiting the website in Safari or any other web browser and follow the simple instruction. Use meme generator websitesīesides the above options, you can also use one of the following websites to create a meme from scratch or edit a meme template with your custom text. From here, tap the meme you just uploaded.Ĩ) Press the meme and tap Download Image to save it to your iPhone’s Photos app. Here’s how to make a custom meme using Imgur on iPhone:ġ) Download Imgur, open it and create a free account.ĥ) Add your desired text to create a custom meme and tap Done.Ħ) Make sure it says “Hidden” and tap Upload.ħ) Tap the profile icon from the bottom right to see your posts. Besides browsing and enjoying the posts there, you can also create a meme from scratch, upload it to Imgur (without making it publicly available) and then save it locally to your iPhone’s Photos app. Imgur is a vibrant community for viral and popular images and memes of all kinds. You can also add stickers and extra text by tapping A+.ħ) When you finish creating, tap Done > Save to have this meme in your iPhone’s Photo Library. Here’s how:ġ) Download GIF & Memes on your iPhone and open it.Ģ) Tap a meme under Hot Memes or Memes DIY.ģ) Select an existing image, GIF, or template.Ħ) Now, double-tap to add your custom text. Besides, you can also pick an image from your Photos app and add text at the top or bottom in different styles. GIF & Memes is another app that lists some of the most famous meme templates that you can edit by adding your custom text and save that as a meme. Plus, the availability of the latest meme templates is a treat that lets you stay updated with what’s in trend. I will go with the Will Smith & Chris Rock meme template.Ĥ) Double-tap to edit the text and add something your creative mind wants.ĥ) Optionally, you can use the other tools to add text, bubbles, image, stickers, etc.Ħ) Finally when done, tap Export > Save to have this meme in your iPhone’s Photos app.īesides that, you can also swipe left and pick one of the meme templates under the Meme Ideas section of the Mematic app. Here’s how to make a meme on iPhone using Mematic:Ģ) Choose Free Style or one of the other templates or styles from the top.ģ) You can now select a meme or stock photo or choose your own image by tapping Photo. Alternatively you can pick a meme idea inside the app and edit it with your custom text. Mematic lets you create memes from scratch by using any image from your Photos app. On the left, I’m using Studio to create a meme, and on the right, I’m using Canva. I downloaded these meme templates from the internet. ![]() After that, I open that image in Studio or Canva, use rectangle shapes to cover the current text and then add my own text to make a personal meme. Some apps allow you to use your own images and share your memes on different social media platforms. These apps are usually straightforward: all you have to do is pick an image, add your own custom text to it, and voila You’ve got yourself a meme. If I find some funny meme or image on Twitter or the internet, I download it to my iPhone. A meme generator is an app that allows you to make your own memes quickly and easily. Using an acquainted app to create memes ensures that you don’t have to download anything new and dig in through its options. All that app needs to be capable of is adding text to an image or letting you put more than one image together in different styles. So, the easiest way to create memes is by using a photo editor app you already use on your iPhone. Anything that conveys the message with some image or text can be considered a meme. The best thing about memes is that there isn’t a fixed format. Use any photo editor you’re familiar with To help you do that, here are a few ways to create memes from scratch or edit a well-known meme template on your iPhone and iPad. If you love memes, you might want to create some with your personal image or text. Write the bottom and top caption and generate your customized meme.Memes lighten the mood and add a fun element to a conversation.Select from our high quality memes gallery.Upload an image from your gallery or take a new photo.With Meme Generator – Create Your Own Memes application, you can create personal memes or a meme from our high quality memes collection WITHOUT ANY WATERMARKS! Offering you the latest options in trend, we have collected the coolest, funniest and most requested meme images on our application! Now, you can caption your favorite characters or photos to create funny moments.Ī quick and easy way to generate a custom meme and caption a photo with original font and style! Memes are a way to say what you think in a funny, humorous, sharp, immediate and realistic way. Sprout sprout- BREED stamp subtract-headings towards towards-nowrap towardsxy towardsxy-nowrap turtle turtles turtles-at turtles-from turtles-here turtles-on turtles-own uphill value-from Patch-related primitivesĭiffuse diffuse4 distance distance-nowrap distancexy distancexy-nowrap inspect is-patch? myself neighbors neighbors4 no-label nobody nsum nsum4 -of patch patch-at patch-ahead patch-at-heading-and-distance patch-here patch-left-and-ahead patch-right-and-ahead patches patches-from patches-own self sprout sprout- BREED value-from Agentset primitivesĪny? ask at-points BREED-at BREED-here BREED-on count histogram-from in-radius in-radius-nowrap is-agent? is-agentset? is-patch-agentset? is-turtle-agentset? max-one-of min-one-of neighbors neighbors4 one-of other-turtles-here other- BREED-here patches patches-from random-n-of random-one-of turtles with with-max with-min turtles-at turtles-from turtles-here turtles-on values-from Color primitivesĮxtract-hsb extract-rgb hsb rgb scale-color shade-of? wrap-color Control flow and logic primitivesĪnd carefully end error-message foreach if ifelse ifelse-value let loop map not or repeat report run runresult (semicolon) set stop startup to to-report wait while without-interruption xor Display primitivesĭisplay no-display no-label screen-edge-x screen-edge-y screen-size-x screen-size-y HubNet primitives Self set-default-shape setxy shapes showturtle Myself no-label nobody -of other-turtles-here other- BREED-here patch-ahead patch-at-heading-and-distance patch-here patch-left-and-ahead patch-right-and-ahead pen-down Run each command, consult each individual entry in the dictionary.īREED-at BREED-here BREED-on clear-turtlesĬreate- BREED create-custom- BREED create-custom-turtlesĭie distance distance-nowrap distancexy distancexy-nowrap downhill downhill4 dx dy forward To see which agent (turtles, patches, observer) can actually ![]() Primitive might still be called by patches or observers, and vice ![]() This dataframe essentially replicates the NetLogo environment, with column labels corresponding to the xcor patch coordinates, and indices following the pycor coordinates.NetLogo 2.1.0 User Manual: Primitives Dictionary Categories of Primitives The patch_report method can be used to return a dataframe which (for this example) contains the countdown attribute of each NetLogo patch. ![]() ![]() Due to the 300 Blackout’s relatively slow speeds, you don’t need super duper premium bullets to do the job on deer and other game animals. Tons of great 30 caliber projectiles are out there that’ll work great in the 300, so you don’t have to buy expensive factory ammunition if that’s all that’s available near you. It’s as simple as rough trimming the case to length, full length sizing with case wax/lube, and then final trimming. Now, if you’re well setup as a reloader and don’t mind forming cases, you should look into reforming. 300 Blackout is $1+ per round in the US and around $1.50-$2 in Canada. Since one is a NATO standard round and has been for decades, while the other is a new (2011 SAAMI acceptance) cartridge, this is to be expected. So, the 223 is much better ballistically, but given that the 300 Blackout is using a bullet over twice the weight with the same case, you were expecting that anyways, right? 223 vs 300 Blackout vs 7.62X39 300 Blackout vs 223 Ammo CostĢ23 is cheaper than 300 Blackout. Not such a big deal when you’re comparing a 1″ drift to a 1.6″ drift at 100 yards in a 10 mph crosswind, but considerably better at 500 yards where the 223 will drift around 30″ compared with the Blackout at 50″. The high speed and short time to target also helps the 223 in wind drift, where it beats the 300 Blackout by around 50%-80% depending on range. That makes getting hits on man sized targets with the 223 at 500 yards fairly easy with a good rest, but considerably more frustrating with the 300 Blackout, especially if you have errors in your ranging. By the time we’re at 500 yards, the 300 Blackout is well past 90″ down compared with a 223 at around 50″, or about half the drop. While the 223 crosses the 10″ down mark at a barely-better 320-340 yards compared with the 275 on the 300 Blackout, it stays much flatter further out. For boar at less than 100 yards, it’d work a treat and they wouldn’t know what hit them if you ran the subsonic rounds with a suppressor. It’s also far lower in energy than I’m comfortable using on a deer. Making an accurate hit at 200 yards with a subsonic round is a bit of fantasy, because you’re looking at 30+ inches of drop even if you sighted at 100 yards. The tougher part to account for on these heavier bullets is the rainbow trajectory. Wow, so little drop in velocity, but I guess that’s what happens when it’s poking along so slowly that wind resistance is minimal. Unless you can range accurately, it’s going to get very tough making hits past 300 yards. Zeroed for 100 yards, you’re at 10″ low by about 275. It generates a bit more energy at the muzzle than 223 and uses sane speeds, so I think it’d be a better close range deer cartridge than the 223. Very similar to ballistics on the 7.62×39, but lagging in power at 100-150 yards. It’s important to separate the 110 grain supersonic round vs the 200+ grain subsonic rounds due to their very different ballistic profiles.Įffectively, it’s a 100 yard cartridge for hunting deer. ![]() 223 magazinesįor a civilian shooter, getting into 300 Blackout when you already have an AR-15 is pretty easy: you can buy a dedicated upper to have some flexibility, or switch to a 300 Blackout barrel for a permanent mod and you’re done. The 300 Blackout was designed with a few things in mind: If only we could get 7.62×39 performance in an AR-15… And then there’s the case taper: which enables great semi automatic reliability but doesn’t work well with the straight magwell and magazines of the AR15. The case head is wide enough that you give up quite a bit of metal on bolt lugs after hogging out the bolt head. 30 in the AR-15? Some manufacturers offer AR-15’s that run 7.62×39, but that cartridge isn’t the best for the AR-15. The AR-10 delivers a lot more power but it’s heavier, gives up magazine capacity, isn’t nearly as common or standardized as the AR-15 platform, and the case capacity is a bit of a waste if you want to run subsonic rounds. Within the Armalite family, there’s the AR-10 in. Let’s dive into the 300 Blackout as it performs against the. It’s easy to convert AR-15’s to and easy to form cases if you’re into reloading. Delivering similar ballistic performance to the 7.62×39, but using a slightly lighter bullet, the 300 Blackout alleviates some hunter’s concerns around bullet mass on game AND makes for a handy suppressed round in an AR-15. ![]() The 300 Blackout, sometimes called the 300 AAC Blackout and somewhat similar to the 300 Whisper®, is what happens when you want an easy 30 caliber conversion for your AR-15. ![]() In some cases, up to 60% of patients treated with checkpoint inhibitors incur severe immune-related toxicities, generally considered to result from reactivation of dormant or suppressed immune responses ( 12). Despite clinical success in reactivating antitumor immune responses, current immune checkpoint blockades targeting PD-1, PD-L1, and CTLA-4 are not exempt from off-target effects and toxicities. Although discovering effective approaches to modulate Treg cells remains a high priority, clinically meaningful progress has been hampered by small molecule or deletional anti-Treg cell-focused therapies lacking sufficient cellular specificity to avoid inadvertent targeting of other T cell populations ( 7– 11). Poor clinical outcomes are predicted by increased peripheral and intratumoral Treg cells in a number of settings, including in patients who receive adoptive cellular therapies and immune checkpoint blockade ( 3– 6). Regulatory T cells (Treg cells) have been a therapeutic target of interest for decades based on early work showing that their depletion amplifies antitumor immune reactivity ( 1, 2). These results demonstrate a proof of concept for rationally designed FOXP3-directed peptide therapeutics that could be used as approaches to amplify endogenous immune responsiveness. We show that lead SAH-FOXP3s bind FOXP3, are cell permeable and nontoxic to T cells, induce dose-dependent transcript and protein level alterations of FOXP3 target genes, impede Treg cell function, and lead to Treg cell gene expression changes in vivo consistent with FOXP3 dysfunction. We describe the design, synthesis, and biochemical evaluation of single- and double-stapled SAHs covering the entire coiled-coil expanse. Using the crystal structure of the FOXP3 homodimer as a guide, we developed SAHs in the likeness of a portion of the native FOXP3 antiparallel coiled-coil homodimerization domain (SAH-FOXP3) to block this key FOXP3 protein-protein interaction (PPI) through molecular mimicry. To overcome dosing limitations and off-target effects from antibody-based Treg cell deletional strategies or small molecule drugging, we investigated the ability of hydrocarbon stapled alpha-helical (SAH) peptides to target FOXP3, the master transcription factor regulator of Treg cell development, maintenance, and suppressive function. Despite continuing advances in the development of novel cellular-, antibody-, and chemotherapeutic-based strategies to enhance immune reactivity, the presence of regulatory T cells (Treg cells) remains a complicating factor for their clinical efficacy. |